interaspecific hybridization of gladiolus grandiflora (hortolanus. hort) using in vitro mature ovule embryo culture

In Vitro Shoot Multiplication and Callus Induction in Gladiolus Hybridus Hort

The present investigation was carried out to study the In vitro shoot multiplication and callogenesis in Gladiolus. For this purpose, cormel, meristem and leaf explants were chosen. Different concentrations of BAP i. e., 0.5, 1.0, 1.5, 2.0 or 2.5 mgL (2.22, 4.44, 6.66, 8.88 or 11.10 μM) were supplemented to MS medium. Both cormel and meristem explants showed maximum multiplication on MS medium ...

Relation between the low temperature stress and catalase activity in gladiolus somaclones (Gladiolus grandiflorus Hort.)

In order to evaluate low temperature tolerance of five variable somaclones as well as the cv. Peter Pears of gladiolus (Gladiolus grandiflorus Hort.) from which they were originated, catalase (CAT) activity and hydrogen peroxide (H2O2) level were measured. Before carrying out the analyses, vitroplants of these clones were exposed, during 21 days, to 8 8C temperature. In response to the stress g...

Intraspecific Hybridization of Populus euphratica Oliv. Using in vitro Technique

Plant Growth Regulators Impact on Vegetative and Reproductive Characteristics of Gladiolus Cut Flowers (Gladiolus hybridus Hort)

Gladiolus is an important marketable cut flower, especially valued for use in floral arrangements. We assessed the effects of two different methods of application (foliar spray and drench) and various concentrations of gibberellic acid (GA3), salicylic acid (SA), mival (MI) and krizatcin (KR) on vegetative and reproductive features of Gladiolus hybridus. The experiment was planned in a factoria...

: the effect of sericin levels (silk glue protein) on rate of in vitro maturation, fertilization and culture of sheep oocytes

هدف از آزمایش اول بررسی اثر سطوح مختلف سریسین [0 (control), 0.1, 0.5, 1.0, 2.5 %] افزوده شده به محیط , ivm بر cumulus cell expansion، بلوغ هسته و توسعه متوالی جنین، در گوسفندان نژاد سنجابی در فصل تولید مثلی می باشد. از سرگیری میوز به وسیله خارج شدن اولین پولار بادی اندازه گیری و هم چنین درصد رسیدن جنین های دو سلولی به مرحله کلیواژ و بلاستوسیت نیز به عنوان نشانه ای از میزان شایستگی توسعه اولیه ج...

Ovule and embryo development, apomixis and fertilization.

Genetic analyses, particularly in Arabidopsis, have led to the identification of mutants that define different steps of ovule ontogeny, pollen stigma interaction, pollen tube growth, and fertilization. Isolation of the genes defined by these mutations promises to lead to a molecular understanding of these processes. Mutants have also been obtained in which processes that are normally triggered ...